The story of Queen, one of the most prominent rock bands ever, began in 1971. Guitarist Brian May and drummer Roger Taylor used to play in Smile, in the beginning, and then started their own project with Freddie Mercury on vocals. In early 1971, they were joined by bassist John Deacon and completed the lineup formation. In late 1972, Queen signed a contract with EMI Records and gave their first performance at the Marquee club in London the following spring.
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. Part of the book series (MIMB, volume 927) Abstract There are multiple clinical situations where cryopreservation of sperm is required including sperm banking prior to chemotherapy or radiation treatment for cancer, donor sperm for couples without a male partner, and various etiologies of male factor infertility. Bunge and Sherman presented the first pregnancy resulting from the thawing of cryopreserved sperm in 1953 (Bunge RJ, Nature 172:767–768, 1953). Starbucks create your own tumbler 16 oz template. Since that time, cryopreservation techniques have continued to improve. Here, we describe protocols for the cryopreservation and thawing of semen and testicular tissues using a simple liquid nitrogen vapor technique.
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